           
|
Objective 1.
To increase by 6-fold the
content and bioavailability of Zn/Fe in cassava roots of a
model cassava cultivar and demonstrate its viability in the
field and efficacy in humans.
Manary, M.J.
- Manary MJ, Hotz C, Krebs NF, Gibson RS, Westcott JE,
Broadhead RL, Hambidge KM (2002) Zinc homeostasis in Malawian
children on a high phytate maize-based diet. Am J
Clin Nutr 75: 1057-1061.
Sayre, R.T.
- Rubinelli P, Siripornadulsil S, Gao-Rubinelli F and Sayre
RT (2002) Cadmium and iron-stress inducible gene expression
in the green alga Chlamydomonas reinhardtii: evidence
for H43 protein function in iron assimilation. Planta 215:
1-13.
Schachtman, D.
- Ramesh, S., Shin, R., Eide, D.J. and Schachtman, D.P.
(2003) Differential metal selectivity and gene expression
of two zinc transporters from rice. Plant Physiology
133: 126-134.
Objective 2.
To increase by 4-fold the
protein content of cassava roots of a model cassava cultivar
and demonstrate its viability in the field and efficacy in
humans.
Fauquet, C. and Taylor, N.
- Speichinger, J., Taylor, N., Fauquet, C. and Chen,
S. (2005). The cassava (Manihot esculenta Crantz)
root proteome: protein identification and differential
expression. Proteomics. In Press.
Manary, M.J.
-
Manary, MJ (2001) Protein -
Energy Malnutrition: There is still work to do. J
Pediatr Gastro Nutr 32: 519-520.
-
Manary MJ, Yarasheski KE, Smith S, Abrams ET, Hart
CA (2004) Protein quantity, not protein quality, accelerates
whole-body leucine kinetics and the acute phase response
during acute infection in marasmic Malawaian children.
Brit J Nutr. 92: 589-595.
-
Manary MJ, Yarasheski KE, Smith S, Abrams ET, Hart
CA (2004) Protein quantity, not protein quality, accelerates
whole-body leucine kinetics and the acute phase response
during acute infection in marasmic Malawaian children.
Brit J Nutr 92:589-595.
-
Manary MJ, Yarasheski KE, Smith
S, Abrams ET, Hart CA (2004) Protein quantity, not protein
quality, accelerates whole-body leucine kinetics and
the acute phase response during acute infection in marasmic Malawaian children.
Brit J Nutr 92:589-595.
Sayre, R.T.
- Siritunga D and Sayre, RT (2004) Engineering cyanogen
synthesis and turnover in cassava (Manihot esculenta).
Plant Molecular Biology 56: 661-669.
Zhang, P. and Gruissem, W.
-
Zhang P, Bohl-Zenger S, Puonti-Kaerlas J, Potrykus I
and Gruissem W (2003) Two cassava promoters related to
vascular expression and storage root formation. Planta
218, 192-203.
-
Zhang P, Jaynes JM, Potrykus I, Gruissem W and Puonti-Kaerlas
J (2003) Transfer and expression of an artificial storage
protein (ASP1) gene in cassava (Manihot esculenta Crantz).
Transgenic Research 12, 243-250.
-
Zhang P, Jaynes JM, Potrykus I, Gruissem W and Puonti-Kaerlas
J (2003) Transfer and expression of an artificial storage
protein (ASP1) gene in cassava (Manihot esculenta Crantz):
towards improving nutritive value of storage roots. In:
Vasil I.K. (ed.) Plant Biotechnology 2002 and Beyond. Kluwer
Academic Publishers, The Netherlands. Pages 425-427.
Objective 3.
To increase by 10-fold the vitamin A and
E content of cassava roots of a model cassava cultivar
and demonstrate its viability in the field and efficacy
in humans.
Cahoon, E.B.
-
Cahoon EB, Hall SE, Ripp KG, Ganzke TS, Hitz WD, Coughlan
SJ (2003) Metabolic redesign of vitamin E biosynthesis
in plants for tocotrienol production and increased antioxidant
content. Nature Biotechnology 21: 1082-1087.
- Sattler SE, Cahoon EB, Coughlan
SJ, DellaPenna D (2003) Characterization of tocopherol
cyclases from higher plants and cyanobacteria. evolutionary
implications for tocopherol synthesis and function. Plant
Physiol. 132: 2184-2195.
Objective 4.
To decrease by 10-fold the cyanogen content
in cassava roots of a model cassava cultivar and demonstrate
its viability in the field.
Sayre, R.T. and Siritunga, D.
-
Mkpong OE, Yan H, Chism G and Sayre RT (1990) Purification,
characterization and localization of linamarase in cassava.Plant
Physiol. 93: 176-181.
-
McMahon J.M and Sayre RT (1993)
Differential biosynthesis of linamarin in low and high
cyanide cultivars of cassava. in: Proceedings of the
First International Scientific Meeting of the Cassava
Biotechnology Network; Roca, W.M. and Thro, A.M. eds.,
pgs. 376-378.
-
Arias-Garzon DI and Sayre RT (1993) Differential
inhibition of transient gene expression in cassava root
and leaf tissues. in: Proceedings of the First International
Scientific Meeting of the Cassava Biotechnology Network; Roca,
W.M. and Thro, A.M. eds., pgs. 239-243.
-
White WLB and Sayre RT (1993) Partial purification
and characterization of hydroxynitrile lyase from cassava.
in: Proceedings of the First International Scientific
Meeting of the Cassava Biotechnology Network; Roca, W.M.
and Thro, A.M. eds., pgs. 379-383.
-
Arias-Garzon DI and Sayre RT (1993) Tissue specific
inhibition of transient gene expression in cassava (Manihot esculenta Crantz)
tissues. Plant Science 93: 121-130.
-
White W, McMahon J and Sayre RT (1994) Regulation of
cyanogenesis in cassava. Acta Horticulturae375: 69-77.
-
McMahon JM, White W, and Sayre RT (1995) Cyanogenesis
in Cassava (Manihot esculenta Crantz). J. Exp.
Bot. 46: 731-741.
-
McMahon J M and Sayre RT (1995) Cyanogenic glycosides:
physiology and regulation of synthesis. In: Phytochemicals
and Health, D.L. Gustine and H.E. Flores, eds.
pgs 75-84.
-
White W and Sayre RT (1995) Characterization of hydroxynitrile
lyase for the production of safe food products from cassava.
In: Phytochemicals and Health, D.L. Gustine
and H.E. Flores, eds. pgs 152-153.
-
Arias-Garzon DI, Sarria R, Gelvin S and Sayre RT (1995)
New Agrobacterium tumefaciens plasmids for cassava
transformation. in: Proceedings of the Second International
Scientific Meeting of the Cassava Biotechnology Network;
Roca, W.M. and Thro, A.M. eds., CIAT, Cali, Colombia,
no. 150, pgs. 245-256.
-
McMahon JM and Sayre RT (1995) Regulation of cyanogenic
potential in cassava (Manihot esculenta Crantz).
in: Proceedings of the Second International Scientific
Meeting of the Cassava Biotechnology Network; Roca, W.M.
and Thro, A.M. eds. CIAT, Cali, Colombia, no. 150, pgs.
423-438.
-
McMahon J and Sayre RT (1997) Partial purification
of the linamarin synthesizing enzyme complex from cassava.
African J. Root Tuber Crops 2: 85-87.
-
White W, Arias-Garzon D, McMahon J and Sayre RT (1998)
Cyanogenesis in cassava: The role of hydroxynitrile lyase
in root cyanide production. Plant Physiology 116: 1219-1225.
-
Sayre RT (1998) Recent Advances in the Biochemistry
and Molecular Biology of Cyanogenesis in Cassava. Proceedings
of the 1st Latin American Conference on Tropical Root
Crops, Sao Paulo, Brazil.
-
Arias-Garzon DI and Sayre RT (2000) Genetic engineering
approaches to reducing the cyanide toxicity in cassava.
(Manihot esculenta, Crantz).In: Cassava
Biotechnology; IV International Scientific Meeting
- CBN. Carvalho LJCB, Thro AM and Vilarinhos
AD eds. Pgs. 213-221. Embrapa, Brasilia.
-
Siritunga D and Sayre RT (2003) Generation of cyanogen-free
transgenic cassava. Planta 217: 367-373.
-
Siritunga D, Arias-Garzon D., White W and Sayre RT
(2004) Over-expression of hydroxynitrile lyase in cassava
roots accelerates cyanogenesis and detoxification. Plant
Biotechnology Journal 2: 37-43.
-
Siritunga D and Sayre, RT (2004) Engineering cyanogen
synthesis and turnover in cassava (Manihot esculenta).
Plant Molecular Biology 56: 661-669.
- Msikita W, Ihemere U, Siritunga D and Sayre RT (2005)
Cassava (Manihot esculenta Cranz) Transformation. In:
Agrobacterium Protocols: Methods in Molecular Biology.
vol. 44. Kan Wang, ed. Humana Press. (in press).
Objective 5.
To delay the rapid post-harvest
physiological deterioration inherent to cassava roots of a
model cultivar and demonstrate its viability in the field.
Beeching, J.
-
Buschmann H, Reilly
K, Rodriguez MX, Tohme J, Beeching JR. (2000) Hydrogen
peroxide and flavan-3-ols in storage roots of cassava (Manihot
esculenta Crantz)
during postharvest deterioration. Journal of Agricultural
and Food Chemistry. 48: 5522-5529.
-
Buschmann H, Rodriguez MX, Tohme
J, Beeching JR. (2000) Accumulation of hydroxycoumarins
during post-harvest deterioration of tuberous roots of
cassava (Manihot esculenta Crantz). Annals
of Botany. 86: 1153-1160.
-
Han Y, Gómez-Vásquez
R, Reilly K, Li H, Tohme J, Cooper RM, Beeching JR. (2001)
Hydroxyproline-rich glycoproteins expressed during stress
responses in cassava. Euphytica.
120: 59-70.
-
Reilly K, Han Y, Tohme J, Beeching
JR. (2001) Isolation and characterisation of a cassava
catalase expressed during post-harvest physiological deterioration. Biochimica
et Biophysica Acta. 1518: 317-323.
-
Cortés DF, Reilly K, Okogbenin
E, Beeching JR, Iglesias C, Tohme J. (2002) Mapping genes
implicated in post-harvest physiological deterioration
in cassava (Manihot esculenta Crantz). Euphytica.
128: 47-53.
-
Reilly K, Gómez-Vásquez
R, Buschmann, H, Tohme J, Beeching JR. (2003) Oxidative
stress responses during cassava post-harvest physiological
deterioration. Plant
Molecular Biology 53: 669-685.
-
Gómez-Vásquez R, Day
R, Buschmann H, Randles S, Beeching JR, Cooper RM. (2004)
Phenylpropanoids, phenylalanine ammonia lyase and peroxidases
in elicitor-challenged cassava (Manihot esculenta)
suspension cells and leaves. Annals
of Botany. 94: 87-97.
- Kemp BP, Beeching JR, Cooper RM.
(2005) cDNA-AFLP reveals genes differentially expressed
during the hypersensitive response of cassava. Molecular
Plant Pathology.
6: 113-123.
Objective 6. To develop a model cassava
cultivar with high levels of resistance to CMD and demonstrate
its resistance in the field.
Fauquet, C. and Taylor, N.
-
Masona, M.V., Taylor, N.J., Robertson, A.I. and Fauquet,
C.M. (2001). Transferring a cassava (Manihot
esculenta Crantz) genetic engineering capability
to the African environment: Progress and prospects. Euphytica,
120: 43-48.
-
Ndjiondjop, M.N., Brugidou, C., Zang, S., Fargette,
D., Ghesquiere, A. and Fauquet, C.M. (2001). High
resistance to rice yellow mottle virus in two cultivated
rice cultivars is correlated with failure of cell to
cell movement. Physiological and Molecular Plant Pathology,
59: 309-316.
-
Pita, J.S., Fondong, V.N., Sangaré, A., Kokora,
R.N.N. and Fauquet, C.M. (2001). Genomic and biological
diversity of the African cassava geminiviruses. Euphytica,
120: 115-125.
-
Pita, J.S., Fondong, V.N., Sangaré, A., Otim-Nape,
G.W., Ogwal, S. and Fauquet, C.M. (2001). Recombination,
pseudorecombination and synergism of geminiviruses are
determinant keys to the epidemic of severe cassava mosaic
disease in Uganda. Journal of General Virology,
82: 655-665.
-
Taylor, N.J., Masona, M.V., Carcamo, R., Ho, T., Schôpke,
C. and Fauquet, C.M. (2001). Production of embryogenic
tissues and regeneration of transgenic plants in cassava
(Manihot esculenta Crantz). Euphytica,
120: 25-34.
-
Fauquet, C.M. (2001). Viral Classification and
Nomenclature. In: Encyclopedia of Life Sciences. London:
Nature Publishing Group. [doi: 10.1038/npg.els.0000440] http://www.els.net/
-
Fofana, I.B.F., Sangaré, A., Ndunguru, J., Kahn,
K. and Fauquet, C.M. (2003). Principles for control
of virus diseases in developing countries. In Gad
Loebenstein and George Thottapilly (Eds.), Virus and
virus-like diseases of major crops in developing countries:
Kluwer Academic, Dordrecht, The Netherlands.
-
Fauquet, C.M., Ceballos, H., and Zhang, P. (Eds.)(2004). Special
Cassava Issue. Plant Molecular Biology.
-
Fauquet, C.M. (2004). Family Geminiviridae. In
Lapierre, H. and Signoret, P.-A. (Eds.), Viruses and
virus diseases of Poaceae (Gramineae). INRA Editions,
Versailles, France.
-
Fauquet, C.M. (2004). Viral classification and
nomenclature. In: Encyclopedia of Life Sciences. London:
Nature Publishing Group. [doi: 10.1038/npg.els.0000440] http://www.els.net/
-
Chellappan, P., Masona, M.V., Vanitharani, R., Taylor,
N.J. and Fauquet, C.M. (2004). Broad spectrum resistance
to ssDNA viruses associated with transgene-induced gene
silencing in cassava. Plant Molecular Biology. 56: 601-611.
-
Fauquet, C.M. and Tohme, J. (2004). The Global Cassava
Partnership for Genetic Improvement. Plant Molecular
Biology. 56:V-X.
-
Fofana, I.B.F., Sangare, A., Collier, R., Taylor, C.
and Fauquet, C.M. (2004). A geminivirus-induced gene
silencing system for gene function validation in cassava.
Plant Molecular Biology. 56: 613-624.
-
Legg, J.P. and Fauquet, C.M. (2004). Cassava
mosaic geminiviruses in Africa. Plant Molecular Biology. 56:585-599.
-
Taylor, N.J., Kent, L. and Fauquet, C.M. (2004). Progress
and challenges for the deployment of transgenic technologies
in cassava. AgBioForum. 7: article 10.
-
Vanitharani, R., Chellappan, P., Pita, J. and Fauquet,
C.M. (2004). Differential roles of AC2 and AC4
of cassava geminiviruses in mediating synergism and post-transcriptional
gene silencing suppression. Journal of Virology 78: 9487-9498.
-
Fauquet, C.M., Mayo, M.A., Maniloff, J., Desselberger,
U. and Ball, L.A. (Eds.)(2005). Virus Taxonomy,
VIIIth Report of the ICTV. Elsevier/Academic Press,
London, pp. 1258.
-
Vanitharani, R., Chellappan, P. and Fauquet, C.M. (2005). RNAi
and gene silencing phenomena mediated by viral suppressors
in plants. In Krishnarao Appasani (Ed.), RNA Interference
Technology: From Basic Science to Drug Development. Cambridge
University, Cambridge, UK.
-
Yadav, J.S., Fauquet, C.M. and Beachy, R.N. (2005). Control
of plant virus diseases. In Robert M. Goodman (Ed.),
Encyclopedia of Plant and Crop Science. Marcel Dekker,
New York. Accepted.
-
Ndunguru, J., Legg, J.P., Thompson, G., Aveling, T.
and Fauquet, C.M. (2005). Molecular biodiversity
of cassava begomoviruses in Tanzania: evolution of cassava
geminiviruses in Africa and evidence for East Africa.being
a center of diversity of cassava geminiviruses. Virology
Journal. 2:21. doi:10.1186/1743-422X-2-21.
-
Ndunguru, J., Legg, J.P., Aveling, T., Thompson, G.
and Fauquet, C.M. (2005). Restriction and sequence
analysis of PCR-Amplified viral DNAs suggests the existence
of different cassava mosaic disease in Tanzania. AAB.
In Press
-
Ndunguru, J., Legg, J.P., Fofana, B., Aveling, T.,
Thompson, G. and Fauquet, C.M. (2005). First report
of a naturally occurring defective interfering molecule
of East African Cassava Mosaic Virus. Archives of Virology.
In Press.
-
Ndunguru, J., Fofana, I.B.F., Legg, J.P., Chellappan,
P., Pita, J.S., Taylor, N.J., Thompson, G., Aveling,
T. and Fauquet, C.M. (2006). Two novel satellite
DNA molecules associated with bipartite cassava mosaic
begomoviruses enhance symptoms and break resistance in
cassava germplasm. Virology. Submitted.
-
Marmey, P. and Fauquet, C.M. (2006). CMI/AAB
Description of Plant Viruses – Cassava vein mosaic
virus. Submitted.
Fregene, M. and Ceballos, H.
-
Fregene M. , Okogbenin E., Marin
J., Moreno I., Ariyo O., Akinwale O., Barrera E., Ceballos
H., and Dixon A. (2006). Molecular Marker Assisted Selection (MAS)
of Resistance to the Cassava Mosaic Disease (CMD). Euphytica
(in press)
-
3. Elizabeth Balyejusa Kizito,
Anton Bua, Martin Fregene, Thomas Egwang, Urban Gullberg
and Anna Westerbergh (2005). The
effect of cassava mosaic disease on the genetic diversity
of cassava in Uganda Euphytica 146:45-54
-
Fregene M., Matsumura H., Akano A., Dixon A., Terauchi
R. (2004). Serial Analysis of Gene Expression (SAGE)
of Host Plant Resistance to the Cassava Mosaic Disease
Resistance (CMD) Plant Molecular Biology 56:563-571.
-
Fregene M., and Mba C. (2004). Marker-Assisted
Selection (MAS). In: Hershey C. (ed). Cassava
Breeding. FAO, Via Caravelle, Rome, Italy
-
Taylor, N., Mkumbira J., Lokko
Y., Fregene M., Fauquet C. (2004) Progress in Understanding,
Generating and Deploying Resistance to Cassava Mosaic Disease. Agronomy
Review (In press)
-
Tomkins J., Fregene M., Main D., Kim H., Wing R., and
Tohme J. (2004). Bacterial Artificial Chromosome (BAC)
Library Resources for Positional Cloning of Pest and
Disease Resistance Genes in Cassava (Manihot esculenta Crantz).
Plant Molecular Biology 56: 555-561
-
Akano A., Barrera E., Dixon A.G.O.,
Fregene M. (2002). Molecular
Genetic Mapping of Resistance to the African Cassava
Mosaic Disease. Theor and Appl Genet 105:521-525.
-
Fregene M.A., Bernal A., Dixon
A., Roca W., and Tohme J.. (2000). AFLP analysis of African
cassava (manihot esculenta crantz) germplasm resistant
to the cassava mosaic disease (CMD). Theor and Appl
Genet. 100:678-685
Zhang and Gruissem
-
Zhang P, Vanderschuren H, Fütterer
J and Gruissem W (2005) Resistance to cassava mosaic disease
in transgenic cassava expressing antisense RNAs targeting
virus replication genes. Plant Biotechnology Journal 3,
385-397.
-
Akbergenov R, Si-Ammour A, Blevins
T, Amin I, Kutter C, Vanderschuren H, Zhang P, Gruissem
W, Meins F Jr, Hohn T, Pooggin MM (2006) Molecular characterization
of geminivirus-derived small RNAs in different plant species.
Nucleic Acids Research 34(2),
462-471
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